Impact of enzymatic digestion on bacterial community composition in CF airway samples

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Impact of enzymatic digestion on bacterial community composition in CF airway samples

BACKGROUND Previous studies have demonstrated the importance of DNA extraction methods for molecular detection of Staphylococcus, an important bacterial group in cystic fibrosis (CF). We sought to evaluate the effect of enzymatic digestion (EnzD) prior to DNA extraction on bacterial communities identified in sputum and oropharyngeal swab (OP) samples from patients with CF. METHODS DNA from 81...

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ژورنال

عنوان ژورنال: PeerJ

سال: 2017

ISSN: 2167-8359

DOI: 10.7717/peerj.3362